Enhanced Delignification of Lignocellulosic Biomass by Recombinant Fungus Phanerochaete chrysosporium Overexpressing Laccases and Peroxidases.
Identifieur interne : 000121 ( Main/Exploration ); précédent : 000120; suivant : 000122Enhanced Delignification of Lignocellulosic Biomass by Recombinant Fungus Phanerochaete chrysosporium Overexpressing Laccases and Peroxidases.
Auteurs : Nancy Coconi Linares [Mexique] ; Francisco Fernández [Mexique] ; Achim M. Loske [Mexique] ; Miguel A. G Mez-Lim [Mexique]Source :
- Journal of molecular microbiology and biotechnology [ 1660-2412 ] ; 2018.
Descripteurs français
- KwdFr :
- Biocarburants (MeSH), Biomasse (MeSH), Cellulose (métabolisme), Clonage moléculaire (MeSH), Dépollution biologique de l'environnement (MeSH), Ergostérol (MeSH), Fibre alimentaire (MeSH), Génie métabolique (MeSH), Laccase (biosynthèse), Laccase (génétique), Lignine (métabolisme), Peroxidases (biosynthèse), Peroxidases (génétique), Phanerochaete (croissance et développement), Phanerochaete (enzymologie), Phanerochaete (génétique), Phanerochaete (métabolisme), Protéines fongiques (génétique), Protéines fongiques (métabolisme), Protéines recombinantes (génétique), Protéines recombinantes (métabolisme), Régulation de l'expression des gènes fongiques (MeSH), Saccharum (MeSH), Transformation génétique (MeSH).
- MESH :
- biosynthèse : Laccase, Peroxidases.
- croissance et développement : Phanerochaete.
- enzymologie : Phanerochaete.
- génétique : Laccase, Peroxidases, Phanerochaete, Protéines fongiques, Protéines recombinantes.
- métabolisme : Cellulose, Lignine, Phanerochaete, Protéines fongiques, Protéines recombinantes.
- Biocarburants, Biomasse, Clonage moléculaire, Dépollution biologique de l'environnement, Ergostérol, Fibre alimentaire, Génie métabolique, Régulation de l'expression des gènes fongiques, Saccharum, Transformation génétique.
English descriptors
- KwdEn :
- Biodegradation, Environmental (MeSH), Biofuels (MeSH), Biomass (MeSH), Cellulose (metabolism), Cloning, Molecular (MeSH), Dietary Fiber (MeSH), Ergosterol (MeSH), Fungal Proteins (genetics), Fungal Proteins (metabolism), Gene Expression Regulation, Fungal (MeSH), Laccase (biosynthesis), Laccase (genetics), Lignin (metabolism), Metabolic Engineering (MeSH), Peroxidases (biosynthesis), Peroxidases (genetics), Phanerochaete (enzymology), Phanerochaete (genetics), Phanerochaete (growth & development), Phanerochaete (metabolism), Recombinant Proteins (genetics), Recombinant Proteins (metabolism), Saccharum (MeSH), Transformation, Genetic (MeSH).
- MESH :
- chemical , biosynthesis : Laccase, Peroxidases.
- chemical , genetics : Fungal Proteins, Laccase, Peroxidases, Recombinant Proteins.
- chemical , metabolism : Cellulose, Fungal Proteins, Lignin, Recombinant Proteins.
- chemical : Biofuels, Dietary Fiber, Ergosterol.
- enzymology : Phanerochaete.
- genetics : Phanerochaete.
- growth & development : Phanerochaete.
- metabolism : Phanerochaete.
- Biodegradation, Environmental, Biomass, Cloning, Molecular, Gene Expression Regulation, Fungal, Metabolic Engineering, Saccharum, Transformation, Genetic.
Abstract
Ligninolytic enzyme production and lignin degradation are typically the rate-limiting steps in the biofuel industry. To improve the efficiency of simultaneous bio-delignification and enzyme production, Phanerochaete chrysosporium was transformed by shock wave-induced acoustic cavitation to co-overexpress 3 peroxidases and 1 laccase and test it on the degradation of sugarcane bagasse and wheat bran. Lignin depolymerization was enhanced by up to 25% in the presence of recombinant fungi in comparison with the wild-type strain. Sugar release on lignocellulose was 2- to 6-fold higher by recombinant fungi as compared with the control. Wheat bran ostensibly stimulated the production of ligninolytic enzymes. The highest peroxidase activity from the recombinant strains was 2.6-fold higher, whereas the increase in laccase activity was 4-fold higher in comparison to the control. The improvement of lignin degradation was directly proportional to the highest peroxidase and laccase activity. Because various phenolic compounds released during lignocellulose degradation have proven to be toxic to cells and to inhibit enzyme activity, a significant reduction (over 40%) of the total phenolic content in the samples treated with recombinant strains was observed. To our knowledge, this is the first report that engineering P. chrysosporium enhances biodegradation of lignocellulosic biomass.
DOI: 10.1159/000485976
PubMed: 29486469
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Loske</name><affiliation wicri:level="1"><nlm:affiliation>Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Querétaro, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Querétaro</wicri:regionArea><wicri:noRegion>Querétaro</wicri:noRegion></affiliation></author><author><name sortKey="G Mez Lim, Miguel A" sort="G Mez Lim, Miguel A" uniqKey="G Mez Lim M" first="Miguel A" last="G Mez-Lim">Miguel A. G Mez-Lim</name><affiliation wicri:level="1"><nlm:affiliation>Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Irapuato, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Irapuato</wicri:regionArea><wicri:noRegion>Irapuato</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of molecular microbiology and biotechnology</title><idno type="eISSN">1660-2412</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biodegradation, Environmental (MeSH)</term><term>Biofuels (MeSH)</term><term>Biomass (MeSH)</term><term>Cellulose (metabolism)</term><term>Cloning, Molecular (MeSH)</term><term>Dietary Fiber (MeSH)</term><term>Ergosterol (MeSH)</term><term>Fungal Proteins (genetics)</term><term>Fungal Proteins (metabolism)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Laccase (biosynthesis)</term><term>Laccase (genetics)</term><term>Lignin (metabolism)</term><term>Metabolic Engineering (MeSH)</term><term>Peroxidases (biosynthesis)</term><term>Peroxidases (genetics)</term><term>Phanerochaete (enzymology)</term><term>Phanerochaete (genetics)</term><term>Phanerochaete (growth & development)</term><term>Phanerochaete (metabolism)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (metabolism)</term><term>Saccharum (MeSH)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Biocarburants (MeSH)</term><term>Biomasse (MeSH)</term><term>Cellulose (métabolisme)</term><term>Clonage moléculaire (MeSH)</term><term>Dépollution biologique de l'environnement (MeSH)</term><term>Ergostérol (MeSH)</term><term>Fibre alimentaire (MeSH)</term><term>Génie métabolique (MeSH)</term><term>Laccase (biosynthèse)</term><term>Laccase (génétique)</term><term>Lignine (métabolisme)</term><term>Peroxidases (biosynthèse)</term><term>Peroxidases (génétique)</term><term>Phanerochaete (croissance et développement)</term><term>Phanerochaete (enzymologie)</term><term>Phanerochaete (génétique)</term><term>Phanerochaete (métabolisme)</term><term>Protéines fongiques (génétique)</term><term>Protéines fongiques (métabolisme)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (métabolisme)</term><term>Régulation de l'expression des gènes fongiques (MeSH)</term><term>Saccharum (MeSH)</term><term>Transformation génétique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Laccase</term><term>Peroxidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term><term>Laccase</term><term>Peroxidases</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulose</term><term>Fungal Proteins</term><term>Lignin</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Biofuels</term><term>Dietary Fiber</term><term>Ergosterol</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Laccase</term><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Laccase</term><term>Peroxidases</term><term>Phanerochaete</term><term>Protéines fongiques</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellulose</term><term>Lignine</term><term>Phanerochaete</term><term>Protéines fongiques</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodegradation, Environmental</term><term>Biomass</term><term>Cloning, Molecular</term><term>Gene Expression Regulation, Fungal</term><term>Metabolic Engineering</term><term>Saccharum</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biocarburants</term><term>Biomasse</term><term>Clonage moléculaire</term><term>Dépollution biologique de l'environnement</term><term>Ergostérol</term><term>Fibre alimentaire</term><term>Génie métabolique</term><term>Régulation de l'expression des gènes fongiques</term><term>Saccharum</term><term>Transformation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Ligninolytic enzyme production and lignin degradation are typically the rate-limiting steps in the biofuel industry. To improve the efficiency of simultaneous bio-delignification and enzyme production, Phanerochaete chrysosporium was transformed by shock wave-induced acoustic cavitation to co-overexpress 3 peroxidases and 1 laccase and test it on the degradation of sugarcane bagasse and wheat bran. Lignin depolymerization was enhanced by up to 25% in the presence of recombinant fungi in comparison with the wild-type strain. Sugar release on lignocellulose was 2- to 6-fold higher by recombinant fungi as compared with the control. Wheat bran ostensibly stimulated the production of ligninolytic enzymes. The highest peroxidase activity from the recombinant strains was 2.6-fold higher, whereas the increase in laccase activity was 4-fold higher in comparison to the control. The improvement of lignin degradation was directly proportional to the highest peroxidase and laccase activity. Because various phenolic compounds released during lignocellulose degradation have proven to be toxic to cells and to inhibit enzyme activity, a significant reduction (over 40%) of the total phenolic content in the samples treated with recombinant strains was observed. To our knowledge, this is the first report that engineering P. chrysosporium enhances biodegradation of lignocellulosic biomass.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29486469</PMID><DateCompleted><Year>2019</Year><Month>06</Month><Day>24</Day></DateCompleted><DateRevised><Year>2019</Year><Month>06</Month><Day>24</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1660-2412</ISSN><JournalIssue CitedMedium="Internet"><Volume>28</Volume><Issue>1</Issue><PubDate><Year>2018</Year></PubDate></JournalIssue><Title>Journal of molecular microbiology and biotechnology</Title><ISOAbbreviation>J Mol Microbiol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Enhanced Delignification of Lignocellulosic Biomass by Recombinant Fungus Phanerochaete chrysosporium Overexpressing Laccases and Peroxidases.</ArticleTitle><Pagination><MedlinePgn>1-13</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1159/000485976</ELocationID><Abstract><AbstractText>Ligninolytic enzyme production and lignin degradation are typically the rate-limiting steps in the biofuel industry. To improve the efficiency of simultaneous bio-delignification and enzyme production, Phanerochaete chrysosporium was transformed by shock wave-induced acoustic cavitation to co-overexpress 3 peroxidases and 1 laccase and test it on the degradation of sugarcane bagasse and wheat bran. Lignin depolymerization was enhanced by up to 25% in the presence of recombinant fungi in comparison with the wild-type strain. Sugar release on lignocellulose was 2- to 6-fold higher by recombinant fungi as compared with the control. Wheat bran ostensibly stimulated the production of ligninolytic enzymes. The highest peroxidase activity from the recombinant strains was 2.6-fold higher, whereas the increase in laccase activity was 4-fold higher in comparison to the control. The improvement of lignin degradation was directly proportional to the highest peroxidase and laccase activity. Because various phenolic compounds released during lignocellulose degradation have proven to be toxic to cells and to inhibit enzyme activity, a significant reduction (over 40%) of the total phenolic content in the samples treated with recombinant strains was observed. To our knowledge, this is the first report that engineering P. chrysosporium enhances biodegradation of lignocellulosic biomass.</AbstractText><CopyrightInformation>© 2018 S. Karger AG, Basel.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Coconi Linares</LastName><ForeName>Nancy</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Irapuato, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fernández</LastName><ForeName>Francisco</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Querétaro, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Loske</LastName><ForeName>Achim M</ForeName><Initials>AM</Initials><AffiliationInfo><Affiliation>Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Querétaro, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gómez-Lim</LastName><ForeName>Miguel A</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Irapuato, Mexico.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>02</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>J Mol Microbiol Biotechnol</MedlineTA><NlmUniqueID>100892561</NlmUniqueID><ISSNLinking>1464-1801</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D056804">Biofuels</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004043">Dietary Fiber</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>11132-73-3</RegistryNumber><NameOfSubstance UI="C036909">lignocellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>9006-97-7</RegistryNumber><NameOfSubstance UI="C027433">bagasse</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.10.3.2</RegistryNumber><NameOfSubstance UI="D042845">Laccase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="D010544">Peroxidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>Z30RAY509F</RegistryNumber><NameOfSubstance UI="D004875">Ergosterol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D056804" MajorTopicYN="N">Biofuels</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="Y">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004043" MajorTopicYN="N">Dietary Fiber</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004875" MajorTopicYN="N">Ergosterol</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D042845" MajorTopicYN="N">Laccase</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060847" MajorTopicYN="N">Metabolic Engineering</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D031786" MajorTopicYN="N">Saccharum</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Biofuels</Keyword><Keyword MajorTopicYN="Y">Lignin degradation</Keyword><Keyword MajorTopicYN="Y">Ligninolytic enzymes</Keyword><Keyword MajorTopicYN="Y">Phanerochaete chrysosporium</Keyword><Keyword MajorTopicYN="Y">Recombinant strains</Keyword><Keyword MajorTopicYN="Y">Shock waves</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>07</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>11</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>2</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>6</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>2</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29486469</ArticleId><ArticleId IdType="pii">000485976</ArticleId><ArticleId IdType="doi">10.1159/000485976</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Mexique</li></country></list><tree><country name="Mexique"><noRegion><name sortKey="Coconi Linares, Nancy" sort="Coconi Linares, Nancy" uniqKey="Coconi Linares N" first="Nancy" last="Coconi Linares">Nancy Coconi Linares</name></noRegion><name sortKey="Fernandez, Francisco" sort="Fernandez, Francisco" uniqKey="Fernandez F" first="Francisco" last="Fernández">Francisco Fernández</name><name sortKey="G Mez Lim, Miguel A" sort="G Mez Lim, Miguel A" uniqKey="G Mez Lim M" first="Miguel A" last="G Mez-Lim">Miguel A. G Mez-Lim</name><name sortKey="Loske, Achim M" sort="Loske, Achim M" uniqKey="Loske A" first="Achim M" last="Loske">Achim M. Loske</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= Enhanced Delignification of Lignocellulosic Biomass by Recombinant Fungus Phanerochaete chrysosporium Overexpressing Laccases and Peroxidases.
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